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Tender Details
- Country: United Kingdom
- Summary: The Development of a Dpcr Assay for American Mink (Neovison Vison)
- GBT Ref No: 106210486
- Deadline: 05 Sep 2024
- Competition: ICB
- Financier: Self Financed
- Purchaser Ownership: Public
- Tender Value: 25000
- Notice Type: Tender
- Document Ref. No.: NE220824DDAAM
- Purchaser's Detail :
- Description :
- The development of a dPCR assay for American mink (Neovison vison).
1. qPCR validation
a. The successful contractor will evaluate four existing assays for American mink that target either the COI gene (Pugh 2022), cyt-b gene (Di Girolamo 2022) or ND2 gene (Takaba and others 2024) using the validation scale for single species eDNA assays (Thalinger and others 2021).
b. Areas for assay improvement should be identified, and further in silico and in vitro testing conducted using qPCR to ensure at least one assay reaches Level 3 minimum on the validation scale (Thalinger and others 2021). The assay(s) should be able to achieve a R-squared value ≥0.990 and efficiency of 90-110%.
c. If these validation criteria are not met, the successful contractor must design a new assay that does meet the validation criteria.
d. A confidence assessment should be undertaken for the best-performing assay following the requirements set out in NECR359 "A framework for assessing confidence in environmental DNA qPCR assays and results" (K.J. Harper and others 2021). This framework (also known as COASTER) requires a standardised data input, as well as user-defined settings, and can be accessed through common web browsers. The tool operates in R under the Shiny framework. A link to the tool will be made available to the successful contractor (it is planned for this to be open access in the near future).
2. ddPCR or dPCR validation
a. The successful contractor will transiti... - Documents :
Purchaser : NATURAL ENGLAND
Lancaster House Hampshire Ct Newcastle upon Tyne NE4 7YH United Kingdom
Contact Person : Annie Ivison
Email :annie.ivison@naturalengland.org.uk
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